Explain about Molecular Pathology .... ? " munipalli akshay paul "
Molecular Pathology is a specialized branch of pathology that focuses on the study and diagnosis of disease through the examination of molecules within organs, tissues, or bodily fluids. It combines aspects of molecular biology, biochemistry, and genetics to understand the mechanisms of disease at a molecular level. Key Concepts in Molecular Pathology: 1. Focus on Molecular Mechanisms : It explores how genetic mutations, changes in gene expression, and other molecular alterations contribute to disease. Particularly useful in cancers, infectious diseases, inherited disorders, and autoimmune diseases. 2. Techniques Used : Polymerase Chain Reaction (PCR) – amplifies DNA sequences. Real-Time PCR (qPCR) – quantifies DNA or RNA in real-time. Next-Generation Sequencing (NGS) – provides detailed genetic information. Fluorescence In Situ Hybridization (FISH) – detects genetic abnormalities in chromosomes. Microarrays – analyze gene expression patterns or detect m...